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vibratome tissue slicer  (Precisionary Instruments)


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    Precisionary Instruments vibratome tissue slicer
    Vibratome Tissue Slicer, supplied by Precisionary Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vibratome+tissue+slicer/tissue+slicer/pm33933676-161-25-28
    Average 90 stars, based on 1 article reviews
    vibratome tissue slicer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Non-canonical NRF2 activation promotes a pro-diabetic shift in hepatic glucose metabolism.
    Article Snippet: .. Real-time qRT-PCR (qPCR) analysis Fresh liver tissue was isolated from the WT or Nrf2 / mice and cut into 150 mm thick sections using a vibratome tissue slicer (Precisionary MOLECULAR METABOLISM 51 (2021) 101243 2021 The Author(s). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Non-canonical NRF2 activation promotes a pro-diabetic shift in hepatic glucose metabolism.
    Article Snippet: .. Real-time qRT-PCR (qPCR) analysis Fresh liver tissue was isolated from the WT or Nrf2 / mice and cut into 150 mm thick sections using a vibratome tissue slicer (Precisionary MOLECULAR METABOLISM 51 (2021) 101243 2021 The Author(s). ..

    Isolation:

    Article Title: Non-canonical NRF2 activation promotes a pro-diabetic shift in hepatic glucose metabolism.
    Article Snippet: .. Real-time qRT-PCR (qPCR) analysis Fresh liver tissue was isolated from the WT or Nrf2 / mice and cut into 150 mm thick sections using a vibratome tissue slicer (Precisionary MOLECULAR METABOLISM 51 (2021) 101243 2021 The Author(s). ..



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    FIGURE 5 Preparation of porcine PCLS. (A) Lungs from slaughtered animals from the abattoir are transported on ice to the laboratory. (B) The cranial lobes of apparently healthy lungs are dissected and filled with 37°C low melting-point agarose through a cannula introduced in the bronchus. Filled lung lobes are kept on ice until the agarose becomes solid. (C) By using a tissue coring tool, cylindrical pieces of lung tissue with a bronchioles in the middle are punched out and cut into approximately 300 µm thin tissue slices by using a tissue slicer or <t>vibratome</t> (here: Krumdieck tissue slicer, model MD 4000-01; TSE Systems, Chesterfield, MO, USA). (D) The tissue slices are transferred to cell culture medium (e.g., RPMI) supplemented with antibiotics and antimycotics and bubbled with a normoxic gas mixture at 37°C to remove the agarose from the airway lumen. (E) PCLS can be maintained for up to two weeks in cell culture medium (e.g., RPMI) supplemented with antibiotics and antimycotics in, e.g., a 24-well plate. Photos provided by D. Schaaf, Institute for Microbiology, University of Veterinary Medicine Hannover, Germany.
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    Image Search Results


    FIGURE 5 Preparation of porcine PCLS. (A) Lungs from slaughtered animals from the abattoir are transported on ice to the laboratory. (B) The cranial lobes of apparently healthy lungs are dissected and filled with 37°C low melting-point agarose through a cannula introduced in the bronchus. Filled lung lobes are kept on ice until the agarose becomes solid. (C) By using a tissue coring tool, cylindrical pieces of lung tissue with a bronchioles in the middle are punched out and cut into approximately 300 µm thin tissue slices by using a tissue slicer or vibratome (here: Krumdieck tissue slicer, model MD 4000-01; TSE Systems, Chesterfield, MO, USA). (D) The tissue slices are transferred to cell culture medium (e.g., RPMI) supplemented with antibiotics and antimycotics and bubbled with a normoxic gas mixture at 37°C to remove the agarose from the airway lumen. (E) PCLS can be maintained for up to two weeks in cell culture medium (e.g., RPMI) supplemented with antibiotics and antimycotics in, e.g., a 24-well plate. Photos provided by D. Schaaf, Institute for Microbiology, University of Veterinary Medicine Hannover, Germany.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Primary cell culture systems to investigate host-pathogen interactions in bacterial respiratory tract infections of livestock

    doi: 10.3389/fcimb.2025.1565513

    Figure Lengend Snippet: FIGURE 5 Preparation of porcine PCLS. (A) Lungs from slaughtered animals from the abattoir are transported on ice to the laboratory. (B) The cranial lobes of apparently healthy lungs are dissected and filled with 37°C low melting-point agarose through a cannula introduced in the bronchus. Filled lung lobes are kept on ice until the agarose becomes solid. (C) By using a tissue coring tool, cylindrical pieces of lung tissue with a bronchioles in the middle are punched out and cut into approximately 300 µm thin tissue slices by using a tissue slicer or vibratome (here: Krumdieck tissue slicer, model MD 4000-01; TSE Systems, Chesterfield, MO, USA). (D) The tissue slices are transferred to cell culture medium (e.g., RPMI) supplemented with antibiotics and antimycotics and bubbled with a normoxic gas mixture at 37°C to remove the agarose from the airway lumen. (E) PCLS can be maintained for up to two weeks in cell culture medium (e.g., RPMI) supplemented with antibiotics and antimycotics in, e.g., a 24-well plate. Photos provided by D. Schaaf, Institute for Microbiology, University of Veterinary Medicine Hannover, Germany.

    Article Snippet: Filled lung lobes are kept on ice until the agarose becomes solid. (C) By using a tissue coring tool, cylindrical pieces of lung tissue with a bronchioles in the middle are punched out and cut into approximately 300 μm thin tissue slices by using a tissue slicer or vibratome (here: Krumdieck tissue slicer, model MD 4000-01; TSE Systems, Chesterfield, MO, USA). (D) The tissue slices are transferred to cell culture medium (e.g., RPMI) supplemented with antibiotics and antimycotics and bubbled with a normoxic gas mixture at 37°C to remove the agarose from the airway lumen. (E) PCLS can be maintained for up to two weeks in cell culture medium (e.g., RPMI) supplemented with antibiotics and antimycotics in, e.g., a 24-well plate.

    Techniques: Cell Culture